"Omicron mutation has existed for a long time-reexamination series"

The Government of Japan has recently announced that if an Omicron strain is detected, it will be considered a "rich" contact (simply on the same flight and rich ??) and will be quarantined in a designated facility.

Yesterday I told you that I am wondering how to detect the Omicron strain (multiple mutations in the gene in the peplomer region).

In order to perform gene analysis, it is first necessary to amplify the gene region of the spike protein using the same principle as PCR.

It has been reported that although the gene sequence (oligomer) called the primer used at this time has been improved from the beginning, it may still not be possible to detect a mutant such as Omicron ("Large swathes of dark-". matter (not-sequenced) in the SARS-Cov2 spike protein in significant number of samples in GISAID --probably due to ARTIC-primer artifacts --which will mask real mutations in these genomic regions, and where / when some mutations arose. ”10.31219/ osf.io/hukgm) (Analysis of the ARTIC version 3 and version 4 SARS-CoV-2 primers and their impact on the detection of the G142D amino acid substitution in the spike protein. BioRxiv preprint doi: https://doi.org / 10.101 / 2021.09.27.461949).

Genetic analysis has also shown that there are gene regions that cannot be captured (gene sequences that produce spike proteins).

The fact that it cannot be detected accurately even by genetic analysis becomes remarkable especially when the amount of virus is low (low amplicon) (Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore. Version 1. bioRxiv. Preprint. 2020 Sep 4.doi: 10.1101 / 2020.09.04.283077) (A proposal of alternative primers for the ARTIC Network's multiplex PCR to improve coverage of SARS-CoV-2 genome sequencing. BioRxiv [Internet]. Biorxiv.org; 2020; Available from: https://www.biorxiv.org/content/10.1101/2020.03.10.985150v2.abstract).

In other words, genetic analysis is a developing test method that still has room for improvement.

Therefore, it was only confirmed that the gene sequence was not completely clarified even if the gene analysis was performed with the suspicion of Omicron.

As of September 2020, there are more than 90,000 new coronavirus gene sequences already in the database (GISAID).

More types of new coronaviruses should now be reported.

It is speculated that what is currently making noise with Omicron is that it is now possible to detect mutant types that could not be detected by genetic analysis until now.

This is because it is unlikely that South Africa will rapidly expand to the entire world in a short period of time.

By the way, the first South African case in which the Omicron mutation was discovered was June 17, 2021 from the database (currently this information has been removed from the database).

Will Omicron, which is said to be highly infectious, wait five months and spread to the whole world?

After 5 months, the original variant of Omicron will already have hundreds of variants.

As we said from the beginning that the new corona pandemic is a PCR pandemic, mutant commotions such as Omicron are also festive commotions (using inaccuracies) brought about by the inaccuracies of the genetic analysis pandemic. , Will be revealed in the future (^ _−) − ☆.